Fig. 2

CBR1 participates in radiation sensitivity of HNSCC cells. a, CBR1 protein expression was evaluated in FaDu and YD10B cells transfected with scrambled or CBR1-specific siRNA. b, Colony forming efficiency of cells transfected for 24 h with scrambled or CBR1 siRNA, prior to IR. Radiation sensitivity was increased by CBR1 knockdown (two-way ANOVA; n = 4; *, P < 0.05; **, P < 0.01; ***, P < 0.001 vs. Scrambled siRNA). c, Colony forming efficiency of cells treated with 25 μM hydroxy-PP-Me (CBR1 inhibitor) before and after IR. CBR1 inhibitor significantly decreased cell survival with IR (two-way ANOVA; n = 4; *, P < 0.05; **, P < 0.01; ***, P < 0.001 vs. control). d, CBR1 protein expression was evaluated in FaDu and YD38 cells transfected with Mock and CBR1/WT vectors. e, Colony forming efficiency of cells transfected for 24 h with Mock or CBR1/WT vectors, prior to IR. Radiation sensitivity was reduced by CBR1 overexpression (two-way ANOVA; n = 4; *, P < 0.05; **, P < 0.01; ***, P < 0.001 vs. Mock)