Fig. 5

IL-32γ decreases NF-κB activity in tumor tissues and skin CSCs. a and b Expression of phosphorylated IKKα/β in cytosol extracts and nuclear translocation of p50 and p65 in the nuclear extracts of DMBA/TPA-induced skin tissues or A431 and SK-Mel-28 CSCs were determined by western blotting. c Expression of p50 and p65 in DMBA/TPA-induced skin tissues or A431 and SK-Mel-28 CSCs were analyzed by immunohistochemistry. Scale bar, 10 μm. n = 4. d DNA-binding activity of NF-κB was determined by EMSA in nuclear extracts of A431 and SK-Mel-28 CSCs