Fig. 7

LAT2 binds to p-mTOR^Ser2448 to upregulate glutamine synthetase and increase the intracellular level of glutamine in pancreatic cancer cells. a The Western blotting results showed that RAD001 could downregulate glutamine synthetase in MIA PaCa-2 and PANC-1 cells. b LAT2 knockout could inactivate mTOR and decrease the interaction between p-mTOR^Ser2448 and glutamine synthetase, resulting in a reduced level of glutamine synthetase expression. c LAT2 knockout downregulated p-mTOR^Ser2448 and glutamine synthetase in PANC-1 cells; both p-mTOR^Ser2448 and glutamine synthetase were localized to the cytoplasm