Fig. 6

RNF38 is a direct target of EBV-miR-BART8-3p. a EBV-miR-BART8-3p and its putative binding sequence in 3’UTR of RNF38 mRNA, and mutations are generated as indicated. b Quantification of RNF38protein expression by Western blotting in CNE-1, SUNE-1 and C666–1 cells. c The relative luciferase activity in HEK293 cells after co-transfection with wild-type (WT) or mutant (MT) RNF38 3’UTR reporter genes and EBV-miR-BART8-3p or control. d Relative RNF38 expression is quantified in 10 normal nasopharyngeal specimens and 19 NPC specimens by qPCR, and GAPDH serves as an internal control. e Spearman’s correlation analysis reveals a negative correlation between the EBV-miR-BART8-3p and RNF38 expression in NPC specimens (n = 19)