Fig. 6

Functional evaluations converge to define the major susceptibility of KS to metabolic impairment. a Response of NSCLC NCI-H1299 derived clones treated with increasing concentrations of 2-DG for 72 h, detected by MTS assay and plotted as dose-response curves. The mean of four independent experiments and SD are shown. Statistical analysis was performed using two-way ANOVA test and Bonferroni post-test for multiple comparisons and is reported in the box. b Doubling times (hours) of KS, K, S and WT NSCLC NCI-H1299 clones cultured in different nutrient combinations. Doubling times were extrapolated from growth curves (Additional file 4: Figure S6) and represented as mean and SD of three independent replicates. Doubling times with a positive value higher than 48 h (the last measured time point) were expressed as > 48. Negative values were not reported. Dashed line highlights standard growth rate. Statistical analysis was performed using one-way ANOVA test and Bonferroni post-test for multiple comparisons. For each clone, each doubling time obtained in the different nutrient-deprived conditions was compared to the doubling time of standard culture conditions. * p-value < 0.05, **** p-value < 0.0001