Fig. 2

MNAT1 increases CRC cell growth and MNAT1-knockdown decreases cell growth. HCT116 and DLD1 cells were transfected with pSIN-MNAT1 respectively. A, MNAT1 expressions in the transfected cells were detected using Western-blotting. B, Viability of MNAT1 transfected-HCT116 (a) and DLD1 (b) cells was measured using MTT. C, Colony formation of the transfected- HCT116 and DLD1 cells was detected using colony formation assay. a, HCT116 with pSIN; b, HCT116 with pSIN-MNAT1; c, Cell colony numbers of the transfected HCT116; d, DLD1 with pSIN; e, DLD1 with pSIN-MNAT1; f, Cell colony numbers of the transfected DLD1. D, HCT116 and DLD1 cells were transfected with pLVX-shMNAT1^#1or pLVX-shMNAT1^#2, respectively. MNAT1 protein (a) and mRNA (b, c) in the transfected cells were detected using Western-blotting or Real-time PCR, respectively. E, Viability of shMNAT1 transfected-HCT116 (a) and DLD1 (b) cells was measured using MTT. F, Motility and invasion of HCT116 cells with shMNAT1s were detected with Boyden chamber invasion assay (a), the motility (b) and invasion (c) cells were counted. G, Motility and invasion of DLD1 cells with shMNAT1s were detected (a), the motility (b) and invasion (c) cells were counted. All experiments were repeated three times. Data are presented as means ±S.D. of three independent experiments and were statistically analyzed using Student’s t test. Scale bar, 100 μm. *, p < 0.05