Fig. 8

MNAT1 exerts tumor-promoting effects through p53 in vivo. A, shscramble-HCT116 and shMNAT1^#1-HCT116 cells were subcutaneously injected into the dorsal flanks of mice, and shscramble-HCT116 served as a control. After 17 days, the mice were euthanized. Representative images are shown (a). After euthanization, the tumors of mice were removed. The removed tumors were weighed (b). B, The tumor growth of shscramble-HCT116 and shMNAT1^#1-HCT116 cells in vivo was calculated by tumor volume. C, MNAT1 and p53 expressions in the implanted tumors were detected using immunohistochemistry (IHC) (a), positive cells were counted in 10 fields of the IHC stained section under microscope (b). *, p < 0.05. D, Schematic illustration of MNAT-promoted CRC tumorigenesis. MNAT1 binds to p53, and mediates p53ubiquitin-degaradation, and increases cell growth and decreases cell apoptosis, finally promotes CRC malignance