Fig. 5

Invasion of wt- and ko-MCA tumor cells. a Wt- and ko-TEX (20 μg/ml) were cocultured with 1 μg matrix proteins for 48 h; matrix protein degradation was evaluated by WB, representative examples are shown; b zymography of wt- and ko-MCA culture supernatant collected after 48 h culture in the absence of FCS; MMP9 and MMP2 are indicated; c wt- and ko-MCA cells were seeded on matrigel; invasion (crystal-violet staining of matrigel embedded cells) and penetration (counting cells at the lower membrane site after crystal-violet staining) was evaluated after 48 h incubation; representative examples and mean % invading±SD / No of penetrating cells±SD (triplicates) are shown; significant differences in invasion and penetration between wt- and ko-MCA tumor cells: *; d ko-MCA cells were seeded on matrigel containing 50 μg/ml TEX. Evaluation was performed as described in (c); mean % invading±SD / No of penetrating cells±SD (triplicates) are shown; significant differences in invasion and penetration in TEX-containing matrigel: s. Tspan8ko and dbko TEX poorly degraded coll I, coll IV and LN332 and gelatin. Invasion of Tspan8ko- and/or CD151ko-MCA tumors is severely reduced, but partly rescued by wt-TEX and weakly by Tspan8ko- or CD151ko-TEX; dbko-TEX are ineffective