Fig. 7

miR-10b-3p promoted tumor growth and metastasis in vivo. a Levels of miR-10b-3p in stable overexpressing KYSE150 cells (Lenti-mimic) and control cells (Lenti-vector). b-d Stable miR-10b-3p overexpressing KYSE150 cells were subcutaneously injected into nude mice to form solid tumors, and representative images of tumor volumes and weights were analyzed by in vivo luciferase imaging on the last day of analysis (n = 5 for each group). e-i The metastatic nodules were observed in the lungs, brains, liver, and bone of mice treated with stable miR-10b-3p overexpressing KYSE150 cells or control vector cells by the vein injection method. j-l, KYSE150 cells were subcutaneously injected into nude mice to form solid tumors and synchronously treated with miR-10b-3p antagomir or miR antagomir NC (n = 5 for each group); a 10-nmol miR-10b-3p antagomir as well as the miRNA negative control in 0.1 ml saline buffer was locally injected into nude mice to treat tumor mass once every 5 days for 5 weeks; tumor weight and volume in nude mice. m Immunohistochemical staining of Ki67 and FOXO3 in tumor tissues dissected from nude mice treated with miR-10b-3p antagomir miR or antagomir NC. n FOXO3 protein expression as measured by immunohistochemical staining in 103 ESCC samples and pair-matched esophageal tissues. o Spearman correlation analysis of the negative correlation between the expression of miR-10b-3p and FOXO3. Each experiment was performed in triplicate. Data are presented as the mean value ± SD. ^●, P < 0.05; ^●●, P < 0.01