Fig. 1
Expression and methylation of CYGB in breast cancer. a Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis of CYGB mRNA expression in breast tumor tissue samples and paired tumor adjacent tissue samples. b Low CYGB expression is correlated with poor ten-year relapse-free survival (RFS) in breast cancer patients. Prognosis data was acquired and analyzed using the Kaplan-Meier Plotter database (www.kmplot.com/analysis/index. php?p=service&cancer=breast). c CYGB mRNA expression and promoter methylation in paired breast cancer tissue samples and non-cancerous breast tissue samples of 36 patients from The Cancer Genome Atlas (TCGA). The data were accessed through cBioPortal (http://www.cbioportal.org/). d RT-PCR and MSP analyses of CYGB mRNA expression and promoter methylation in breast cancer cell lines. Non-tumorigenic mammary epithelial cell lines MCF10A and HMEC as well as normal breast tissue samples were used as controls. GAPDH was detected as an input control. e RT-PCR and MSP indicate demethylation by Aza and TSA (A + T) restored CYGB expression in BT549, MB231, MCF7 cells. GAPDH was detected as an input control. Aza: 5-aza-2′-deoxycytidine; BN: breast normal tissue; BrCa: breast cancer tissue; M: methylated; MSP: methylation-specific polymerase chain reaction; RT-PCR: reverse transcription-polymerase chain reaction; U: unmethylated