Fig. 1
The relationship between IMPDH2 expression and poor prognosis of CRC. (a and b) The relative expression of IMPDH2 protein and mRNA in normal human colon epithelial cells (FHC) and seven CRC cell lines (HCT116, SW620, M5, SW480, HT29, DLD-1 and LoVo) by western blotting and qPCR. Mean ± SD (n = 3). (c) The expression of IMPDH2 protein in 8 surgical CRC tissues and their paired adjacent normal tissues by western blotting. (d) The expression of IMPDH2 mRNA in 34 pairs of fresh CRC tissues and matched adjacent normal tissues using qPCR. (e) The IMPDH2 expression in CRC tissues with or without metastases. nmCRC denotes CRC tissues without metastases (n = 18); mCRC denotes CRC tissues with lymph node metastases (n = 16). (f) The expression of IMPDH2 mRNA in 97 paired human CRC tissues and their adjacent normal mucosa tissues from TCGA dataset. IMPDH2 expression was normalized to GAPDH and expressed relative to the match adjacent normal tissues. (g) Representative images of IMPDH2 expression in CRC tissues and paired adjacent normal tissues (NT) by immunohistochemistry, Scale bars, 200 μm and 50 μm, respectively. (h and i) Kaplan-Meier survival analysis of the association between IMPDH2 expression and overall survival or progression-free survival in 214 CRC patients