Fig. 1

SIRT6 silencing blocks TGF-β1-induced EMT in NSCLC cells. a Western blot analysis of SIRT6 protein in A549 and H1299 cells transfected with indicated constructs. One representative experiment of three independent experiments are shown. b Morphological analysis of A549 and H1299 cells transfected with transfected with control (shCtrl) or SIRT6 shRNA (shSIRT6) with or without TGF-β1 treatment. Scale bar = 60 μm. c Real-time PCR analysis of E-cadherin (E-cad) and vimentin mRNA levels in A549 and H1299 cells transfected with indicated constructs after TGF-β1 treatment. Mean fold change was calculated from three independent experiments performed in triplicate, with respect to shCtrl-transfected cells arbitrarily assigned a value of 1. ^*P < 0.05. d Western blot analysis of E-cad and vimentin protein levels in A549 and H1299 cells treated as in (c). One representative experiment of three independent experiments are shown. Numbers indicate fold change in protein levels. e Immunofluorescence analysis of E-cad and vimentin. Scale bar = 20 μm