Fig. 5

KLF4 mediates SIRT6 depletion-induced tumor suppression in vitro. a Western blot analysis of KLF4 protein in A549 and H1299 cells transfected with control or KLF4 siRNA. One representative experiment of three independent experiments are shown. b Transwell invasion assay was used to assess the invasiveness of cells transfected with indicated constructs. Data shown are mean from three independent experiments performed in triplicate. ^*P < 0.05. c Western blot analysis of KLF4 protein in A549 and H1299 cells transfected with empty vector or KLF4-expressing plasmid. One representative experiment of three independent experiments are shown. d Transwell invasion assay was used to assess the invasiveness of cells transfected with indicated constructs. Data shown are mean from three independent experiments performed in triplicate. ^*P < 0.05. e Western blot analysis of E-cadherin and vimentin proteins in A549 and H1299 cells transfected with empty vector or KLF4-expressing plasmid after TGF-β1 treatment. One representative experiment of three independent experiments are shown