Fig. 1
From: RER1 enhances carcinogenesis and stemness of pancreatic cancer under hypoxic environment
RER1 is upregulated in pancreatic cancer tissues and correlated with poor survival outcome. (a and b) IHC analysis of RER1 expression levels in human primary PC specimens and non-tumorous pancreas specimens. The representative pictures were shown at 200× magnification. (c and d) Western blotting and qRT-PCR analysis were performed to test the expression of RER1 in six PC cell lines (AsPC-1, Mia PaCa-2, SW1990, Capan-2, CFPAC-1, and PANC-1) and a normal human pancreatic ductal cell line (hTERT-HPNE). (e) The expression level of RER1 was significantly increased in PC tissues compared with normal tissues from GSE28735. (f) qRT-PCR analysis of RER1 expression in 50 PC tissues and 15 normal human pancreas tissues (shown as log10 (2-ΔCT)). (g) qRT-PCR analysis of RER1 expression in 31 PC tissues from lymph node metastasis (LNM+) patients compared to 19 PC tissues from lymph node metastasis free (LNM-) patients (h) Kaplan–Meier’s analysis of the correlation between RER1 expression and the overall survival of PC patients. (i) Kaplan–Meier’s analysis of the correlation between RER1 expression and the relapse free survival of PC patients. *P < 0.05; **P < 0.01 (χ2 test for b, student’s t-test for others)