Fig. 2

IATL induces oxidative stress in prostate cancer cells. a-b Intracellular ROS generation induced by increasing doses of IATL was measured in PC-3 or DU145 cells by staining with DCFH-DA (10 μM) and flow cytometry analysis. c-d Cells were pre-incubated with 5 mM NAC for 2 h before exposure to IATL. Intracellular ROS generation was measured by flow cytometry. e-f Cells were pre-incubated with 5 mM NAC for 2 h before exposure to IATL for 24 h, cell viability was determined by MTT assay. g-h Cells were pre-incubated with 2000 U/mL catalase for 2 h before exposure to IATL for 24 h, cell viability was determined by MTT assay. i-l Cells were pre-incubated with 5 mM NAC for 2 h before exposure to IATL for 24 h, percentage of cell apoptosis was determined by Annexin-V/PI staining and flow cytometry. The results shown are representative of at least three independent experiments. m-n Cells were pre-incubated with 5 mM NAC for 2 h before exposure to IATL for 20 h. Caspase-3 or caspase-9 activity in the cell extracts was determined by an assay kit using specific substrate