Fig. 4

ER stress contributes to IATL-induced apoptosis in prostate cancer cells. a PC-3 cells were infected with CHOP siRNA or control siRNA, the mRNA expression of CHOP was analyzed by qRT-PCR after stimulation with IATL (40 μM) for 6 h. b PC-3 cells transfected with CHOP siRNA or control siRNA were treated with IATL (40 μM) for 12 h, the protein level of CHOP was determined by western blot. c PC-3 cells transfected with CHOP siRNA or control siRNA were treated with IATL (40 μM) for 20 h, caspase-3 activity in the cell extracts were determined by an assay kit using specific substrate. d PC-3 cells transfected with CHOP siRNA or control siRNA were treated with IATL (40 μM) for 24 h, percentage of cell apoptosis was determined by Annexin-V/PI staining and flow cytometry. The results shown are representative of at least three independent experiments