Fig. 6

Treatment of DNMTi and HDACi reprogram EMT partially mediated by EpCAM and WNT signaling. a Western blotting show up-regulation of E-cadherin and down-regulation of TCF4 by the treatment of DNMTi and HDACi. The number below the band indicates the relative expression level to the control quantified by Ly-Cor Odyssey software. b Representative images of immunofluorescence staining of EpCAM. Magnification, 400×. Scale bar, 20 μm. c Quantification of nuclear EpCAM shows reduced nuclear EpCAM intensity in the cells treated with DAC, SGI, SAHA, MS275, and Tub, whereas an increased intensity was observed in the cells treated with JNJ and LBH