Fig. 2

FXR1 bound with MIR17HG and promotes the effect of MIR17HG on glioma. a MIR17HG was identified in the FXR1 complex. Relative enrichment of MIR17HG was measured using real-time qPCR. Data represent mean ± SD (n = 3 in each group). **P < 0.01 versus anti-normal IgG group, using Student’s t test. b FXR1 and GAPDH protein levels in immunoprecipitation with MIR17HG RNA were evaluated by western blots. The expression levels of FXR1 and GAPDH proteins are shown. c Real-time qPCR analysis for FXR1 regulating MIR17HG expression in U87 and U251 cells. Data are presented as the mean ± SD (n = 3 in each group). **P < 0.01 versus sh-NC group. d The graph represents the relative levels of the MIR17HG at the different actinomycin D treatment times in the control group, sh-NC group, and sh-FXR1 group. e Click-iT Nascent RNA capture kit (Life Technology) was conducted to label and capture newly synthesized RNA, and nascent MIR17HG was measured using real-time qPCR. f CCK-8 assay was conducted to investigate the effect of FXR1 and MIR17HG inhibition on proliferation in U87 and U251 cells. g Flow cytometry analysis of U87 and U251 cells treated with inhibition of FXR1 and MIR17HG. h Quantification number of migration and invasion cells treated with inhibition of FXR1 and MIR17HG. Representative images and accompanying statistical plots were presented. Data are presented as the mean ± SD (n = 3 in each group). *P < 0.05, **P < 0.01 versus sh-FXR1-NC + sh-MIR17HG-NC group (empty vector); ^#P < 0.05, ^##P < 0.01 versus sh-FXR + sh-MIR17HG-NC group; ^△P < 0.05, ^△△P < 0.01 versus sh-FXR1-NC + sh-MIR17HG group. Scale bars represent 40 μm. Using one-way analysis of variance for statistical analysis