Fig. 6

Osthole inhibits MDA-MB-231 xenograft growth in vivo. a Tumor volume in vehicle- and osthole-treated mice. MDA-MB-231 cells were injected in the flanks of mice and tumors were allowed to develop for approximately 8 d (50–150 mm³). Mice bearing MDA-MB-231 xenografts received osthole at 100 or 200 mg/kg interperitoneally [*P < 0.05]. b Images of resected tumor tissues at day 48. Two mice (one in 100 mg/kg osthole group and the other in 200 mg/kg osthole group did not have a visible tumor after 48 d treatment. c Tumor weights determined on day 48 [*P < 0.05 compared to vehicle control]. d Western blot analysis of P-STAT3 levels in resected tumor specimens. GAPDH was used as loading control. e Immunohistochemical staining of tumor sections for cell proliferation marker Ki-67, apoptosis markers Bcl-2 and cleaved caspase-3, and cell cycle markers MDM2, and CDC2. Representative images are shown