Fig. 2

ARNTL is downregulated in nasopharyngeal carcinoma due to its promoter hypermethylation. a Quantitative RT-PCR analysis of ARNTL mRNA expression in NP69 and nasopharyngeal carcinoma cell lines. b ARNTL mRNA is downregulated in the GSE12452 nasopharyngeal carcinoma dataset. c Western blotting assay of ARNTL protein expression in nasopharyngeal carcinoma cell lines and normal NP69. d Western blotting assay of ARNTL protein expression in nasopharyngeal carcinoma tissues (T, n = 4) and normal nasophrynx epithelial tissues (T, n = 4). e ARNTL methylation levels before and after DAC treatment in NP69 and nasopharyngeal carcinoma cell lines. f ARNTL mRNA expression before and after DAC treatment in NP69 and nasopharyngeal carcinoma cell lines. * indicated P < 0.05