Fig. 4

Effects of NF-κB on 14–3-3η and MDR in HCC cells: a Bel/5-Fu cells were treated by 20 μM of ML-385, topotecan, stattic, or BAY-117082 for 24 h. qRT-PCT analyses in triplicate of the 14–3-3η mRNA. b to e Bel/5-Fu cells were transfected by scrambled or NF-κB/p65-siRNA. b qRT-PCT analyses in triplicate of the NF-κB/p65 and 14–3-3η mRNAs. c, top Western blot analyses of the expressions of p-p65 and 14–3-3η. c, bottom) Southwestern blot analyses of the binding of probe (containing the κB sequence located in 14–3-3η promoter) to NF-κB/p65. d Densitometric analysis. e The cell viability was determined in triplicate, and the IC_50s were calculated