Fig. 2
qRT-PCR validation of ten differentially expressed circRNAs in 20 pairs of CRC samples. a The relative expression levels of 5 up-regulated and 5 down-regulated circRNAs (selected from the top 10 dysregulated circRNAs) in 20 pairs of CRC and adjacent normal mucosa tissues. b Sanger sequencing confirmed the back-splice junction sites of circRNAs. c. Comparison of log2FC in ten differentially expressed circRNAs between RNA-Seq and qRT-PCR. Data are shown as means ± s.d. of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001. NS, not significant. T, CRC tumor tissues; N, adjacent normal mucosa tissues