Fig. 7

Overexpression of STAT3 rescued the effects of depletion of IL-8 combined with GEN on M2 polarization of THP-1 macrophages induced by co-culture. THP-1 macrophages were treated with IL-8 depletion of Co-CM from SKOV3-derived OCSLCs co-cultured with THP-1 macrophages transduced with adenovirus expressing STAT3 and in the presence or absence of GEN. Ad-STAT3: The cells transduced with adenovirus expressing STAT3. The levels of CD163 (a) and p-STAT3 (b) expression in THP-1 macrophages as well as the contents of IL-10 and IL-12 (c), NO (d), and IL-8 (e) in Co-CM were shown.*P < 0.05, vs treatment with depletion of IL-8. ^# P < 0.05, vs co-treatment with depletion of IL-8 and GEN (10.0 μM). These experiments were performed in triplicate