Fig. 2
From: ANKHD1 is required for SMYD3 to promote tumor metastasis in hepatocellular carcinoma
ANKHD1 is a co-regulator with SMYD3 and is associated with poor prognosis of HCC. a Lysates of huh7-SMYD3 and huh7-control cells were immunoprecipitated for endogenous H3K4me3 or ANKHD1 and immunoblotted for ANKHD1 or H3K4me3, respectively. b Subcellular fractions were isolated to analyze the coexpression of SMYD3, ANKHD1 and H3K4me3 in cytoplasm and nucleus using western blot. c Confocal analysis of HCCLM3 and huh7 cells transfected with SMYD3 displaying ANKHD1 (green), H3K4me3 (red) and DAPI (blue) staining; MERGE shows the overlapped images. d IHC analysis of SMYD3 expression in HCC tissues. e Kaplan-Meier analysis of the overall survival or recurrence-free survival in HCC patients according to ANKHD1 expression