Fig. 8

XAG suppresses tumor growth in mouse HCC xenograft model. a Mice were respectively given vehicle (0.9% sodium chloride plus 1% DMSO) orally, and injected intraperitoneally with 40 or 80 mg/kg XAG, tumor volume and body weight (e) were measured every 3 days for 24 days. **p<0.01. b After tumor was isolated from mice, the weight of tumor tissues was measured. **p<0.01. c TUNEL staining measured cell apoptosis in tumor tissues. **p<0.01. d IHC detected the expression levels of Ki-67, cleaved caspase-3, Beclin 1, CHOP, GRP78, p-JNK, p-c-jun in tumor tissues. LC3 expression in tumor tissues was detected by Immunofluorescence. **p<0.01. f The levels of ALT, AST, and urea nitrogen in tumor tissues were detected using all-automatic biochemical analyzer. **p<0.01. g HE staining examined the histopathologic characteristics of lung, liver, spleen, kindey, heart in each treatment groups