Fig. 7

Ethanol administration, STARD10 and ERBB2 overexpression promote breast cancer cell malignancy in MCF-7 cells. MCF-7 cells were treated with 100 mM ethanol and transfected with STARD10 and/or ERBB2 for 48 h (a, b) MTT assay showing Data are expressed as (mean ± SE) from 3 to 4 independent experiments performed in triplicates. *p < 0.05 vs. EV. c Migration assay. Results are shown as total wound area at 0 h and 48 h. Data are expressed as (mean ± SE) from 4 independent experiments performed in triplicates. *p < 0.05 vs. EV 48 h. d Pathway schematic depicting known intracellular signaling mechanisms activated downstream of the ethanol administration proposed to mediate cell proliferation as well as cell migration through STARD10 and ERBB2 activation