Fig. 1

NAC decreases protein levels of Notch2, Notch3 signaling and restrains cell proliferation of GBM. a, Cell viability was analyzed by CCK8 at 450 nm. U87 and U251 cells were treated with NAC (2, 5, 10 or 20 mM) for 24 h. b and c The protein levels of Notch2, Notch3, Hes1 and Hey1 were analyzed by western blot. U87 and U251 cells were treated with NAC (2, 5, 10 or 20 mM) for 24 h (b) and NAC (10 mM) for 3, 6, 12, 24 or 48 h (c) respectively. d The protein levels of Notch1 were detected in U87 and U251 cells by western blot after NAC (10 mM) treatment for 24 h. β-actin was used as a loading control. e and f Cell viability was analyzed by CCK8 at 450 nm. U87 (e) and U251 (f) cells were transfected with Scramble, si-Notch2 or si-Notch3 (10 μM) respectively for 24 h and 48 h. Scramble served as a control. All data are presented as means ± SD of three independent experiments. * P < 0.05 compared with control group or Scramble group