Fig. 4
Ultrasound pre-treatment enhances significantly cellular NP-DNA complexes uptake in cancer cells but not normal cells. Cancer HT29, HCT116 and normal HF, E926 cells were plated (5.0x105cells / dish) in 60 mm dishes, then twenty-four hours later, growth media was replaced with OPTIMEM, delivered with liploplex-LUC complexes and thereafter ultrasounds treated for the indicated times. Cells were collected 48 h after treatments. DNA transduction efficiency was evaluated by luciferase assays and values were normalized to protein content and relative LUC activity quantified respect to control set to 1.0. Each experiments have been repeated three times in triplicate, means and standard deviation of representative experiments are reported. Significance was assessed by one-way ANOVA and Dunnett’s multiple comparisons post-hoc test. *P < 0.05, **P < 0.001, ***P < 0.0001