Fig. 6
miR-135b-5p increased doxorubicin-sensitivity of MCF-7 cells in vivo. MCF-7 cells, stably transduced with lentivirual vector expressing double strand precursors of a control miRNA or miR-135b, were inoculated subcutaneously into BALB/c Nude mice at a dose of 5 × 106 per mouse (n = 4 in each group). Mice were injected intraperitoneally with PBS or doxorubicin (5 mg/kg) once on day 12, 15 and 18 after cell inoculation (indicated by arrows in panel A). a Tumor size was measured continuously. b-f On day 32, tumors were collected from mice. c Proteins extracted from tumors were analyzed by western blotting with indicated antibodies. d Histological analysis was performed using H&E staining. e Immunohistochemistry was performed to detect Ki-67-expression in tumor tissues. Scale bar in (d and e): 500 μm. f Percent of Ki-67-positive cells was counted. Experiment was repeated twice. Data are shown as mean ± SD, compared using one-way ANOVA test. *, p < 0.05; n.s., no significance