Fig. 5
PCK1 inhibits hepatoma growth in a subcutaneous xenograft mouse model. MHCC-97H cells were infected with AdGFP or AdPCK1 for 24 h. Control MHCC-97H cells and infected cells (1 × 105 cells/injection) were injected into the flank of nude mice (n = 6 per group). a Tumor growth curves of subcutaneous xenograft tumor model mice. Tumor volumes were calculated as M × (square of W)/2. *P < 0.05 by two-tailed unpaired Student’s t-test. Mice were sacrificed 38 days after injection, followed by xenograft tumor dissection. b Representative images of xenografts tumors. c Tumor size and d tumor weight of subcutaneous xenografts. e PCK1, pAMPK, and p27Kip1 protein expression in tumor tissue samples as detected by western blotting. Relative levels of the indicated proteins were quantified using ImageJ software. **P < 0.01