Fig. 6
Metformin suppresses tumor growth in an orthotopic implantation tumor model established with PCK1-KO hepatoma cells. PCK1-KO and parental cells (1 × 105 cells/injection) were implanted into the left lobes of nude mice livers (n = 5 per group). One week after implantation, mice were treated with metformin (250 mg/kg per day, intraperitoneally) or PBS (equal volume, intraperitoneally) for 6 weeks. The mice were sacrificed 7 weeks after implantation. a Schematic overview of the experiment design. b Tumor foci number in the liver of each mouse. c Representative gross appearance of murine livers. Red arrows: metastatic foci. d, e Representative d HE staining and e IHC detection of PCK1 and pAMPK in liver tumor tissues. Magnification: 100×, 200×