Fig. 4

Upregulation of NUSAP1 promotes an EMT-like phenotype in cervical cancer cell lines. a GSEA plot showing that NUSAP1 expression positively correlates with EMTin published cervical cancer gene expression profiles (TCGA). b EMT markers, such as E-cadherin and vimentin, were analyzed by western blotting in the indicated cells. GADPH was used as the loading control. c, d Immunofluorescence staining for E-cadherin and vimentin in the indicated cells (× 200)