Fig. 6
From: miR-190 enhances endocrine therapy sensitivity by regulating SOX9 expression in breast cancer
ERα and ZEB1 competitively bind to the miR-190 promoter and regulate its expression. a, Promoter analysis of miR-190. Two E-boxes and a half ERE were located on the core promoter region of miR-190 (− 300 to + 1). b, Interaction between ERα or ZEB1 and the miR-190 promoter sequence in MDA-MB-231 and T47D cells as determined by ChIP assay. c, Interaction between ZEB1 or ERα and the miR-190 promoter sequence in ERα-transfected MDA-MB-231 cells (left), ZEB1-transfected T47D cells (right), as well as in control cells as determined by ChIP assay. d, miR-190 promoter activity was measured in cells as in (c) by luciferase analysis. e, Mutation of E-box or/and ERE sequence prevents ZEB1 or ERα from regulating the miR-190 promoter. f, miR-190 expression in cells as in (c) was determined by RT-qPCR. *P < 0.05