Fig. 1

CYT997 inhibited cell proliferation and induced apoptosis in human osteosarcoma cells. a. 143B, SJSA, U2OS, and MG63 osteosarcoma cell lines were treated with CYT997 (0, 20, 40, 80, 160 and 320 μM) for 24 and 48 h. Cell viability was measured by CCK-8 assays. b. 143B and SJSA cells treated with CYT997 (0, 40, 80, 160 μM). Colony formation was evaluated by colony formation assays. c-d 143B and SJSA cells were treated with CYT997 for 24 h and analyzed using PI/Annexin V-FITC flow cytometry. Histograms indicate the proportion of apoptotic cells from three separate experiments. e Cells were treated with various concentrations of CYT997 for 24 h, and apoptosis-related proteins such as cleaved PARP, and caspase-4 were analyzed by western blotting. *P < 0.05, significantly different compared with the control group