Fig. 5

Lamin A/C is SUMOylated in response to DNA damage. MDA-MB-231 cells were treated without or with DOX (10 μM) for 24 h. (a) Nuclear extracts were prepared and immunoprecipitated using anti-lamin A/C, and immunoblotted using anti-SUMO1. (b) Immunofluorescence analysis revealed colocalization of lamin A/C (red) and SUMO1 (green). Scale bars: 10 μm. (c) Immunofluorescence analysis showed a colocalization of SUMO1 (red) with EGFP-LC3 (green), lamin A/C (white) and leaked nuclear DNA (blue). Scale bars: 10 μm