Fig. 8

Knockdown of UBC9 attenuates SUMOylation of lamin A/C and nucleophagy mediated by DNA damage. MDA-MB-231 cells stably expressing sh-Control and sh-UBC9 (1# and 3#) were treated with DOX (10 μM) for 24 h. (a) The expression of UBC9 in sh-Control and sh-UBC9 cells was detected by western blot. (b) Nuclear extracts were prepared, immunoprecipitated using anti-lamin A/C, and immunoblotted using anti-SUMO1. (c) The colocalization of lamin A/C (red), SUMO1 (green) and leaked DNA (blue) was determined by immunofluorescence. Scale bars: 10 μm. (d) Nuclear extracts were prepared, immunoprecipitated with anti-LC3, and immunoblotted with anti-lamin A/C. (e) The colocalization of EGFP-LC3 (green) and lamin A/C (red) was detected by immunofluorescence. Scale bars: 10 μm. (f) The colocalization of EGFP-LC3 (green) and leaked DNA (blue) was detected by immunofluorescence. Scale bars: 10 μm