Fig. 5
Pancreatic tumorigenesis is restrained by knockdown of AFAP1-AS1 or enhanced by inhibition of miR-384 (n = 6). a, picture of tumor formation in nude mice transferred with miR-384 inhibitor or shRNA-AFAP1-AS1; b, tumor volume of nude mice transferred with miR-384 inhibitor or shRNA-AFAP1-AS1; c, tumor weight of nude mice transferred with miR-384 inhibitor or shRNA-AFAP1-AS1; d, expression of miR-384, AFAP1-AS1 and ACVR1 in the mice tumor tissues as determined by RT-qPCR; e and f, the protein expression of ACVR and CSC markers in the mice tumor tissues as measured by western blot analysis; *, p < 0.05 vs. the shRNA-NC group. All the above data were measurement data and expressed as mean ± standard derivation. The data of B was analyzed by repeated measurement analysis, and one-way ANOVA was applied for others. The experiment was repeated three times. PC, pancreatic cancer; miR-384, microRNA-384; AFAP1-AS1, actin filament-associated protein 1 antisense RNA 1; NC, negative control; RT-qPCR, reverse transcription quantitative polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ACVR1, activin receptor A type I; ABCG2, ATP-binding cassette subfamily G member 2