Fig. 6
From: Tobacco smoke induced hepatic cancer stem cell-like properties through IL-33/p38 pathway
Long term TS exposure induced EMT-like alterations in liver tissues of mice. Mice were exposed to TS for 12 weeks, and the expression of E-cadherin, ZO-1, N-cadherin, and Vimentin was detected by immunohistochemical staining (a). b-e Fold changes of E-cadherin (b), ZO-1 (c), Vimentin (d) and N-cadherin (e)-positive area in TS group relative to FA group. (f) Western blotting of E-cadherin, ZO-1, Vimentin and N-cadherin in liver tissues. β-actin was served as the loading control. g The indicated proteins relative to β-actin were assessed by densitometric analysis; six animal samples per group were used for the densitometric analysis. Data are expressed as mean ± SD. The significance was assessed with unpaired two-tailed Student’s t test. # P < 0.05, ## P < 0.01, compared with FA control. FA = filtered air; TS = tobacco smoke