Fig. 3

CTSL crucially mediated the IR-induced EMT in the presence of mut-p53. a The levels of CTSL and EMT-associated transcription factors were examined by western blotting in three p53 status cell lines under IR or not (left panel). The corresponding quantities of protein expression were shown (right panel). b Cells were examined by ChIP assay with or without IR treatment. The recruitment of p53 to the CTSL promoter was shown (up panel). The corresponding quantities of CTSL promoter expression were shown (down panel). c Expression and intracellular location of CTSL in three p53 status cell lines under/ or not IR was determined by immunofluorescence assay. Red bar, 10 μm d) Effects of CTSL siRNA and IR on EMT-associated Zeb1 and Snail protein expressions in Flag-p53 (R273H) H1299 cells by western blotting analysis. e) PCR assay was used to analyze the mRNA level of Zeb1 and Snail in Flag-p53 (R273H) H1299 cells with or without IR. f) The expression of CTSL was analyzed by western blotting in various lung cancer cells with the relatively common mutation spot 175, 248 and 273 of p53. Data are shown as mean ± S.D., n = 3, *P < 0.05, **P < 0.01, *** P < 0.001, Student’s t-test