Fig. 2

miR-205 impairs cell repair of radiation-induced DNA damage. (a) Representative immunofluorescence images of nuclear γH2AX foci (cell nuclei: blue; γH2AX foci: green) in DU145 cells at 1, 4 and 8 h after exposure to 4 Gy of irradiation. (b) Kinetics of γH2AX foci resolution in Neg- or miR-205-reconstituted DU145 cells, expressed as mean number of cells containing > 10 γH2AX foci at 1, 4 and 8 h after exposure to 4 Gy of irradiation (IR). Data are reported as mean ± SD values from 3 independent experiments. (c) (Left panel) Photomicrographs showing formation of Comets in Neg- or miR-205-transfected DU145 cells at 4 h after exposure to 4 Gy of irradiation. (Right panel) Quantification of tail moments counted in at least 200 cells per group, reported as mean ± SD values from 3 independent experiments. The level of significance was represented as ***p < 0.001, Student’s t-test