Fig. 6

LAMP3 downregulation recapitulates miR-205 induced radiosensitizing effect. (a) Surviving fractions at 2 Gy of DU145 cells transfected with either miR-205, siPKCε, siZEB1, siLAMP3 or siRAB27A are reported as mean ± SD values from 3 independent experiments. The level of significance was represented as **p < 0.01, ***p < 0.001, Student’s t-test. (b) Western blot analysis, and corresponding relative quantification, showing LAMP3 (upper panel) and RAB27 (lower panel) protein amount in DU145 cells upon transfection with siLAMP3 or siZEB1, respectively. Vinculin was used as equal protein loading controls. Cropped images of selected proteins are shown