Fig. 6

KDM5B bound to SNHG1 and formed a positive feedback loop to promote the biological behavior of glioma cells. a Sequence-based prediction of KDM5B-SNHG1 interaction, with random forest, RF classifier score of 0.8 and support vehicle machine, SVM classifier score of 0.99. * Interaction probabilities generated by RPISeq range from 0 to 1. In performance evaluation experiments, predictions with probabilities > 0.5 were considered “positive” indicating that the corresponding protein and RNA are likely to interact. b Interaction strength based on interaction propensity rank of binding regions in the positive set using catRAPID strength. c qRT-PCR displayed positive correlation between KDM5B and SNHG1. Data were presented as the mean ± SD (n = 5, each group). **P < 0.01 vs. sh-NC group. d RIP assay with anti-IgG, anti-KDM5B or 10% input from cell extracts. Relative expression of SNHG1 in KDM5B relative to normal IgG immunoprecipitates were determined by qRT-PCR. **P < 0.01 vs. anti-IgG group. e RNA-pulldown assay was performed using in vitro synthesized biotinylated SNHG1. Precipitation reactions were conducted using streptavidin beads and then subjected to Western blot. f SNHG1 RNA half-life measured by qRT-PCR after Actinomycin D treatment. Data were presented as the mean ± SD (n = 3, each group). *P < 0.05 vs. sh-NC group