Fig. 1
PRMT5 and PRMT1 regulate the CFLARL protein level and sensitivity to chemotherapeutic agents. a The indicated cells were seeded into a 6-well cell culture plate and transfected the following day with PRMT5 siRNAs for 48 h. b The indicated cells were seeded into a 6-well culture plate and transfected the following day with the pcDNA3.1 and pcDNA3.1-PRMT5 plasmids for 24 h. The cells were harvested for the preparation of the whole-cell protein lysates and subsequent western blot analysis. c The indicated cells were seeded into a 6-well cell culture plate and transfected with PRMT5 siRNAs on the second day. After 24 h, the cells were seeded again to ensure that the wells contained identical cell densities. Then, the cells were treated with 2 μmol/L doxorubicin for 24 h. The cells were harvested for the preparation of the whole-cell protein lysates and subsequent western blot analysis. d The indicated cells were seeded into a 6-well culture plate and transfected the following day with the pcDNA3.1 and pcDNA3.1-PRMT5 plasmids for 24 h. Then, the cells were treated with 2 μmol/L doxorubicin for 24 h. The cells were harvested for the preparation of the whole-cell protein lysates and subsequent western blot analysis. d Cells were seeded into a 6-well cell culture plate and transfected the following day with PRMT1 siRNAs for 48 h. f Cells were seeded into a 6-well culture plate and transfected the following day with the pcDNA3.1 and pcDNA3.1-PRMT1 plasmids for 24 h. The protein expression of CFLARL was calculated as described in (A). g The indicated cells were seeded into a 6-well cell culture plate and transfected with PRMT1 siRNAs on the second day. After 24 h, the cells were seeded again to ensure that each well contained identical cell densities. The protein expression of CFLARL was calculated as described in (c). Then, the cells were treated with 2 μmol/L doxorubicin for 24 h. h Cells were seeded into a 6-well culture plate and transfected the following day with the pcDNA3.1 and pcDNA3.1-PRMT5 plasmids for 24 h. Then, the cells were treated with 2 μmol/L doxorubicin for 24 h. The cells were harvested for the preparation of the whole-cell protein lysates and subsequent western blot analysis. ACTB or GAPDH was used as a loading control