Fig. 6

PRMT5 and PRMT1 affect NSCLC cell apoptosis induced by doxorubicin. a H460 and H1299 cells were seeded into 6-well cell culture plates and transfected with the PRMT5 plasmid on the second day. After 24 h, the cells were seeded again to ensure that each well contained identical cell densities. The cells were then treated with 2 μmol/L doxorubicin for 24 h. The cells were harvested for the preparation of the whole-cell protein lysates and subsequent western blot analysis. GAPDH expression was detected as a loading control. b A549 and H1299 cells were seeded into 6-well cell culture plates and transfected with PRMT5 siRNA on the second day. After 24 h, the cells were seeded again to ensure that each well contained identical cell densities. Then, the cells were treated with 2 μmol/L doxorubicin for 24 h. The cells were harvested for the preparation of the whole-cell protein lysates and subsequent western blot analysis. c H157 and H460 cells were seeded into 6-well cell culture plates and transfected with the PRMT1 plasmid on the second day. After 24 h, the cells were seeded again to ensure that each well contained identical cell densities. Then, the cells were treated with 2 μmol/L doxorubicin for 24 h. The cells were harvested for the preparation of the whole-cell protein lysates and subsequent western blot analysis. d H460 and H1299 cells were seeded into 6-well cell culture plates and transfected with PRMT1 siRNA on the second day. After 24 h, the cells were seeded again to ensure that each well contained identical cell densities. Then, the cells were treated with 2 μmol/L doxorubicin for 24 h. The cells were harvested for the preparation of the whole-cell protein lysates and subsequent western blot analysis. GAPDH expression was detected as a loading control