Fig. 7
PRMT5 and PRMT1 affect NSCLC cellular apoptosis by regulating the protein levels of CFLARL. a A549-LacZ cells and A549-CFLARL cells were seeded into 6-well cell culture plates and transfected with PRMT5 siRNA on the second day. After 24 h, the cells were seeded again to ensure that each well contained identical cell densities. Then, the cells were treated with 2 μmol/L doxorubicin for 24 h. The cells were harvested for the preparation of the whole-cell protein lysates and subsequent western blot analysis. b A549-LacZ cells and A549-CFLARL cells were seeded into 6-well cell culture plates and transfected with the pcDNA3.1-PRMT1 plasmid on the second day. After 24 h, the cells were seeded again to ensure that each well contained identical cell densities. Then, the cells were treated with 2 μmol/L doxorubicin for 24 h. The cells were harvested for the preparation of the whole-cell protein lysates and subsequent western blot analysis. c H460 cells and A549 cells were transfected with control siRNA or PRMT5 siRNA. After 48 h, the cells were harvested and lysed for the western blot analysis. D H460 cells and A549 cells were transfected with control siRNA or PRMT1 siRNA. After 48 h, the cells were harvested and lysed for the western blot analysis