Fig. 8
PRMT5 competes with PRMT1 for binding to CFLARL. a H1299 cells were transfected with the pEBG-GST-CFLARL or pcDNA3.1-MYC-PRMT1 plasmids and co-transfected with pcDNA3.1 or pcDNA3.1–PRMT5. Then, the cells were treated with 20 μmol/L MG132 for 4 h. The cells were harvested and prepared for the GST pull-down assay. The protein levels of CFLARL and MYC were detected by western blotting. b Cells were transfected with the pEBG-GST-CFLARL or pcDNA3.1-MYC-PRMT1 plasmids and co-transfected with control siRNA or PRMT5 siRNA. Regarding the subsequent experiments, refer to (A). c Cells were transfected with the pEBG-GST-CFLARL or pcDNA3.1-MYC-PRMT5 plasmids and co-transfected with pcDNA3.1 or pcDNA3.1–PRMT1. Then, the cells were treated with 20 μmol/L MG132 for 4 h. The cells were harvested and prepared for the GST pull-down assay. The protein levels of CFLARL and MYC were detected by western blotting. d Cells were transfected with the pEBG-GST-CFLARL or pcDNA3.1-MYC-PRMT5 plasmids and co-transfected with control siRNA or PRMT1 siRNA. Regarding the subsequent experiments, refer to (C). e Summary of PRMT5-regulated and PRMT1-regulated apoptosis signaling pathways in NSCLC cells