Fig. 5

SOX13, a target of miR-138-5p, regulated the GEC angiogenesis in U87 glioma. (a) A putative binding site between SOX13 and miR-138-5p was predicted and the relative luciferase activity was expressed as the firefly/renilla luciferase activity. Values represent the means ± SD (n = 5, each group). ^**P < 0.01 vs. SOX13 Wt + miR-138-5p(+)NC group. (b-c) The mRNA and protein expression levels of SOX13 were measured in ECs and GECs by qRT-PCR and western blot. Values represent the means ± SD (n = 5, each group). ^**P < 0.01 vs. ECs group. (d) SOX13 regulated the viability of GECs. Values represent the means ± SD (n = 5, each group). ^**P < 0.01 vs. SOX13(+)NC group; ^##P < 0.01 vs. SOX13(−)NC group. (e) SOX13 regulated the migration of GECs. Values represent the means ± SD (n = 5, each group). ^**P < 0.01 vs. SOX13(+)NC group; ^##P < 0.01 vs. SOX13(−)NC group. The scale bar represents 100 μm. (f) SOX13 regulated the tube formation of GECs. Values represent the means ± SD (n = 5, each group). ^*P < 0.05, ^**P < 0.01 vs. SOX13(+)NC group; ^##P < 0.01 vs. SOX13(−)NC group. The scale bar represents 100 μm. (g-h) The mRNA and protein expression levels of SOX13 were inhibited after knockdown of circ_002136. Values represent the means ± SD (n = 3, each group). ^*P < 0.05, ^**P < 0.01 vs. circ_002136(−)NC group. (i-j) The mRNA and protein expression levels of SOX13 were regulated by miR-138-5p. Values represent the means ± SD (n = 3, each group). ^**P < 0.01 vs. miR-138-5p(+)NC group. ^##P < 0.01 vs. miR-138-5p(−)NC group. (K-L) The mRNA and protein expression levels of SOX13 were co-regulated by both circ_002136 and miR-138-5p. Values represent the means ± SD (n = 3, each group). ^**P < 0.01 vs. circ_002136(−)NC + miR-138-5p(+)NC group