Table 1 Primers and probes used for RT-qPCR
From: FUS/circ_002136/miR-138-5p/SOX13 feedback loop regulates angiogenesis in Glioma
Primer or Probe | Gene | Sequence (5′- > 3′) or Assay ID |
|---|---|---|
Primer | FUS | F: GCCAAGATCAATCCTCCATGAGTAGTG |
R: TCCACGGTCCTGCTGTCCATAG | ||
circ_002136 | F: CTTTCCGAGACATTTGCTGG | |
R: CATGGAGATCACAATAAGGAACTC | ||
P:FAM + TCTTCTTCTCCTCTGTCTTCC+MGB | ||
GAPDH | F: GGACCTGACCTGCCGTCTAG | |
R: TAGCCCAGGATGCCCTTGAG | ||
P:FAM + CCTCCGACGCCTGCTTCACCACCT+Eclipse | ||
CDK11A | F: AGAGGAAGAGGAGGAGGAGGAGAC | |
R: CGAACCGTGACTCTGGAACAACC | ||
SOX13 | F: CTGGACTTCAACCGAAATTTGA | |
R: GTTCCTTCCTAGAAACCTCTCC | ||
SPON2 | F: GATTGTAGACAGCGCCTCAGTTCC | |
R: GACGCACTCAGCCTCTTCTTCG | ||
Probe | MiR-138-5p U6 | 002284(Applied Biosystems) |
001973(Applied Biosystems) |