Fig. 3
From: PRIMA-1MET-induced neuroblastoma cell death is modulated by p53 and mycn through glutathione level
Protein and gene expression after treatment with PRIMA-1MET. a: Results of protein expression measured by ICW demonstrate that 6 h of 60 μM PRIMA-1MET does not induce phosphorylation of ATM or p53 in NB cell lines (dark grey: non-treated vehicle control, light grey: PRIMA-1MET, dots: outliers). There is no significant upregulation of p21 or Bax due to PRIMA-1MET treatment. b: Results of gene expression measured by real-time PCR demonstrate that major p53 target genes involved in cell cycle arrest (14–3-3, Gadd45, p21) remain unchanged (dark grey: non-treated vehicle control, light grey: PRIMA-1MET, dots: outliers). Bax is involved in signaling apoptosis and did not show any changes in levels as measured by ICW (A) or real-time PCR (B). Noxa was consistently and significantly upregulated and showed on average a 2-fold increase. CXCR4, a marker of aggressive NB, was downregulated after PRIMA-1MET treatment. c: BE-2C and SK-N-DZ (both MNA cell lines) showed accumulation of TP53 mRNA. d: BE-2C, CHP212, NGP and SK-N-DZ have high expression of MYCN, which is in accordance with the previously-described presence of MNA in these cell lines