Fig. 5
From: SHMT1 inhibits the metastasis of HCC by repressing NOX1-mediated ROS production
Increased ROS production is responsible for the functional influence of SHMT1 knockdown on Hep3B cells. (a) DCFH-DA fluorescence assay was used to investigate the effect of SHMT1 knockdown on ROS production. H2O2 treatment was used as positive control of cellular ROS production. (b) DCFH-DA fluorescence assay was used to examine whether NAC treatment abrogated the increase of ROS production induced by SHMT1 knockdown. (c) Boyden-chamber and transwell assay were performed to investigate whether NAC treatment abrogated the increase of cell migration and invasion induced by SHMT1 knockdown. (d) and (e) qRT-PCR and western blot were employed to investigate whether NAC treatment abrogated the promoting effect of SHMT1 knockdown on EMT. *P < 0.05